Mouse Genotype

There are three types of genotype:

1. Complete genotype.

The genotyping work is done and the genotype is complete.

2. Incomplete genotype.

The genotyping work is done but the genotype is incomplete because there are still loci where the genotype is unknown.

For example, a mouse from a cross Etv4+/-;Etv5+/-;Tcre X Etv4+/-;Etv5F/F may carry Tcre, Etv4-, Etv5- and Etv5F alleles, thus several PCR reactions are required to know its full genotype. If you do a Cre PCR first and find out that this mouse doesn't have Tcre, you may stop doing any further genotyping because it is not a mutant you need. You can set the genotype of the mouse as Etv5F and select the "Incomplete Genotype" option. The mouse will not show up in the genotyping list any more.

Incomplete genotype shown as "(" + genotype + ")". Example: (Etv5F).

3. Genotyping in progress.

The genotyping work is still ongoing. The mouse is still listed on the genotyping list.

In the previous example, if you do a Cre PCR first and find out that this mouse has Tcre, then you may need to do more genotyping to get the full genotype, that may take a while. You can enter the available genotyping result by selecting the "Genotyping in Progress" option. By this way the mouse remains in the genotyping list, and you can always come back to update its genotype and change the status to "complete".

Genotyping in progress shown as "UC(" + genotype + ")". Example: UC(Tcrei).

You can update the genotype in several ways:

1. Update genotype in "Mouse detail" window by clicking "Genotype" button. In the next window, single click allele node to select the right allele. If you are not sure about the PCR result, uncheck the "Sure" box. Unsure genotype shown as "{" + genotype+ "}". Example: {Etv4+/-};Etv5F/F.

 

The genotype will be added into the dropdown list in "Mouse detail" window so that you can directly select for next records.

For wild type mice, set genotype from the dropdown list.

2. From the genotyping list, select the mice and open the "Update genotype" window. You can open the genotyping list from "Today's tasks" window or "Task calendar" window. Recommended for updating lots of mouse records at one time.

In the "Update Genotype" window, click "Add Alleles" to add alleles. At most four alleles from one gene can be added. You also can set genotype as "wild type", "control", "mutant" or "unknown" from the drop down list. The default type of genotype is "Uncompleted".

 

3. Update from "Genotyping result" window. See Update Genotype from PCR Result for detail.